TNF is secreted by macrophages,monocytes,neutrophils,T-cells,NK-cells following their stimulation by bacterial LPS.Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha.The synthesis of TNF-alpha is induced by many different stimuli including interferons,IL2,GM-CSF.TNF-β is a potent mediator of inflammatory and immune responses.It belongs to the TNF family of ligands,and signals through TNFR1 and TNFR2.TNF-β is produced by activated T and B lymphocytes,and has similar activities to TNF-α.It mediates a large variety of inflammatory,immunostimulatory,and antiviral responses.
Recombinant Human TNF-β produced in E.coli is is a glycosylated polypeptide chain of 172 amino acids.A fully biologically active molecule,rhTNF-β has a molecular mass of 18.6kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Species：……………………………Human
Source：………………………………E.coli
Measured Molecular Weight：……18.6kDa,observed by reducing SDS-PAGE.
Purity：………………………………> 95% as analyzed by SDS-PAGE.
Formulation：………………………Lyophilized after extensive dialysis against PBS.
Reconstitution：……………………Reconstituted in ddH2O or PBS at 100μg/ml.
氨基酸序列：
MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK
QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV
VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK
MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL
SPSTVFFGAF AL
Lyophilized recombinant Human Tumor Necrosis Factor-beta (TNF-β) remains stable up to 6months at lower than -70℃ from date of receipt.Upon reconstitution,Human TNF-β should be stable up to 1 week at 4℃ or up to 3 months at -20℃.For long term storage it is recommended that a carrier protein (example 0.1% BSA) can be added.Avoid repeated freeze-thaw cycles.
ED50 <4pg/mL,measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D,corresponding to a specific activity of>2.5×108 units/mg.
相关搜索：人TNFβ/TNFSF1，Recombinant Human TNF-β